Determination of Quercetin in Yuxingcao Dispensing Granule by HPLC

LIANG Jie; LIU Xian-fu; SUN Zheng-yi; YU Jing

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Determination of Quercetin in Yuxingcao Dispensing Granule by HPLC

更新时间：2024-01-04

- Determination of Quercetin in Yuxingcao Dispensing Granule by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 11, Pages: 140-143(2012)
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- Published：2012
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LIANG Jie, LIU Xian-fu, SUN Zheng-yi, et al. Determination of Quercetin in Yuxingcao Dispensing Granule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 140-143.
DOI：

LIANG Jie, LIU Xian-fu, SUN Zheng-yi, et al. Determination of Quercetin in Yuxingcao Dispensing Granule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 140-143. DOI：

摘要

目的: 建立测定鱼腥草配方颗粒中槲皮素含量的HPLC方法。方法: 采用正交试验优化鱼腥草配方颗粒的提取工艺

用HPLC测定鱼腥草配方颗粒中槲皮素的含量。色谱柱为Hypersil ODS C18柱(4.6 mm×250 mm

5 μm)

流动相为甲醇-0.2%磷酸(45 :55)

检测波长372 nm

流速1.0 mL·min-1

柱温30 ℃。结果: 槲皮素在0.09~1.44 μg线性关系良好(r=0.999 9)

平均加样回收率为100.6%

RSD 1.68%(n=6)。结论: 该方法简便、快速、重复性好

可作为鱼腥草配方颗粒中槲皮素的含量测定方法。

Abstract

Objective: To establish an HPLC method for determination of quercetin in Yuxingcao dispensing granules. Method: Orthogonal experiments were used to optimize extraction technology. The samples were separated on the Hypersil ODS C18 (4.6 mm×250 mm

5 μm) column which was eluted with methanol-0.2% phosphoric acid solution (45 : 55)with detective wavelength at 372 nm and flow rate at 1 mL·min-1

column temperature at 30 ℃. Result: The method had good linear relationship within the range of 0.09-1.44 μg(r=0.999 9)for quercetin. The average recovery rate was 100.6% with RSD of 1.68%. Conclusion: The method is simple

rapid and reliable which can be used to determin quercetin in Yuxingcao dispensing granules.

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