Influence of Buzhong Yiqi Tang on Myocardial Apoptosis and Expression of Proteins Fas, FasL and Caspase-3 in Rats of Hypothyroidism
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Influence of Buzhong Yiqi Tang on Myocardial Apoptosis and Expression of Proteins Fas, FasL and Caspase-3 in Rats of Hypothyroidism
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 10, Pages: 236-240(2012)
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Published:2012
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GAO Tian-shu, HAN Xiao-qing, YIN Hui-si. Influence of Buzhong Yiqi Tang on Myocardial Apoptosis and Expression of Proteins Fas, FasL and Caspase-3 in Rats of Hypothyroidism[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(10): 236-240.
DOI:
GAO Tian-shu, HAN Xiao-qing, YIN Hui-si. Influence of Buzhong Yiqi Tang on Myocardial Apoptosis and Expression of Proteins Fas, FasL and Caspase-3 in Rats of Hypothyroidism[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(10): 236-240.DOI:
Influence of Buzhong Yiqi Tang on Myocardial Apoptosis and Expression of Proteins Fas, FasL and Caspase-3 in Rats of Hypothyroidism
Objective: To study the effect of Buzhong Yiqi Tang(tonifying middle-Jiao and Qi treatment) on myocardial apoptosis and expression of proteins Fas
FasL and Caspase-3 in hypothyroid rats. Method: The rat of hypothyroidism models were prepared by Kolaja method.Healthy wistar rats were randomly divided into normal control group
model group
Buzhong Yiqi Tang group
Letrox(L-T4)group. The morphological and ultrastructural changes were observed under optical microscope and electronic microscope respectively. Apoptotic cells were measured by TdT-mediated dUTP nick end labeling (TUNEL) method.The expression of apoptosis-related proteins Fas
FasL and Caspase-3 were detected with immunohistochemical methods. TSH was measured by ELISA
Totel 3 Triodothy ronine(TT3)and Totel 4 Triodothy ronine (TT4) were measured by RIA;detect the iodine in urine. Result: Compared with control group
the apoptosis index and the expression of Fas
FasL and Caspase-3 reached the highest level in the model group
myocardial cell injured severely. After treatment the apoptosis index of Buzhong Yiqi Tang group(12.10±0.73) and L-T4 group(29.26±2.10) decreased
the expression of Fas
FasL and Caspase-3 also reduced
and the effectiveness of Buzhong Yiqi Tang group was better(P<0.05). Conclusion: Hypothyroidism causes increase of myocardial cell apoptosis
heightened expression of Fas
FasL and Caspase-3
while Buzhong Yiqi Tang might decrease the myocardial apoptosis and lower the expression of protein Fas
FasL and Caspase-3 significantly
it indicated that Buzhong Yiqi Tang has protective action on myocardial injury of hypothyroidism.
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Related Institution
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