Comparison of Contents of Four Alkaloids in Different Processed of with Quantitative Analysis Multi-components by Single Marker Method
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Comparison of Contents of Four Alkaloids in Different Processed of with Quantitative Analysis Multi-components by Single Marker Method
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 18, Pages: 11-13(2012)
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Published:2012
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ZHAO Jun-ying, WANG Kun, ZHANG Zhen-ling. Comparison of Contents of Four Alkaloids in Different Processed of with Quantitative Analysis Multi-components by Single Marker Method[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 11-13.
DOI:
ZHAO Jun-ying, WANG Kun, ZHANG Zhen-ling. Comparison of Contents of Four Alkaloids in Different Processed of with Quantitative Analysis Multi-components by Single Marker Method[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 11-13.DOI:
Comparison of Contents of Four Alkaloids in Different Processed of with Quantitative Analysis Multi-components by Single Marker Method
Objective: To compare the contents of epiberberine
coptisine
berberine and palmatine in different processed of Coptis chinensis. Method: Quantitative analysis multi-components by single marker was used to determine the the contents of four alkaloids in different processed C. chinensis.Chromatographic conditions were:Agilent TC-C18 column(2) (4.6 mm×250 mm
5 μm)
mobile phase of acetonitrile-0.05 mol · L-1 potassium dihydrogen phosphate(50: 50
plus 0.4 g sodium dodecyl sulfate per 100 mL
phosphate-regulating pH 4.0)
detection wavelength 345 nm
column temperature 25℃
flow rate 1 mL · min-1
number of theoretical plates calculated to berberine hydrochloride should be greater than 2 000. Result: The contents of epiberberine
coptisine
berberine and palmatine in different processed of C. chinensis had different degrees of reduction. Conclusion: Established determination method in this test was rapid and economical
it could be used as quality testing method for different processed of C. chinensis.
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Related Author
ZHAO Jun-ying1
WANG Kun3
ZHANG Zhen-ling3
JIA Xiu-mei
QIN Xinpeng
ZOU Wei
MENG Ying
LIU Sicong
Related Institution
河南中医学院
河南中医学院第二附属医院
Henan Province Hospital of Traditional Chinese Medicine
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