Objective: To establish an method for determination of hydroxysafflor yellow A

gallic acid and aristolochic acid A in Wuwei Zhaxun pill. Method: The hydroxysafflor yellow A

gallic acid and aristolochic acid A in Wuwei Zhaxun pill was determined by HPLC. CAPCELL PAK C18(4.6 mm×250 mm

5 μm)column was used and detection wavelength was at 403

273

390 nm. The mobile phase was methanol-0.4% phosphoric acid (30: 70)

methanol-0.2%phosphoric acid (7: 93)

methanol-0.05% glacial acetic acid (79: 21)

with the flow rate of 1.0 mL · min-1.Result: The calibration curves of hydroxysafflor yellow A

gallic acid and aristolochic acid A were in a good linearity over the ranges of 0.116-1.740 μg (r=0.999 97)

0.067-0.673 μg(r=0.999 95)

0.024-0.247 μg(r=0.999 99)

respectively. The average recoveries were 98.62%(RSD 2.15%)

100.01%(RSD 2.03%)

96.83%(RSD 1.74%). Conclusion: The method is simple

accurate and can be used for the control.