Objective : To observe the influence of the hemp seed oil on the related parameters of skin in the aging mice

and study the anti-aging function of the fructus cannabis. Method : D-galactose was given by hypodermic injection to copy the subacute aging model of the mice. 1 h later

corresponding drugs were given to their groups for 42 days. The influence of hemp seed oil of high

medium

and low doses (12.0

6.0

3.0 mL·kg-1) on superoxide dismutase(SOD)

malondiadehyde(MDA)

lipofuscin(Lpf)

hydroxyproline(Hyp) and skin moisture content were observed

respectively. Histopathological observation of mice skin including the epidermal and dermal thickness of the mice skin at the same part of the body was observed. The difference between dermal collagen fibers arrays of different experimental groups was compared

and the area and morphological changes of the elastic fibers were examined. Result: The average contents of MDA and Lpf in skin homogenates of large dose group were 7.13 nmol·mg-1and 0.83 μg·g-1

the average contents of MDA in skin homogenates of medium dose group were 7.29 nmol·mg-1

all of these indexes were decreased significantly compared with which of model group(P<0.05).The average activitis of SOD in skin homogenates of large & medium dose groups were 21.95 U·mg-1 and 21.45 U·mg-1

the Hyp contents of them were 8.31 μg·mg-1 and 7.92 μg·mg-1

all of these indexes were increased significantly compared with which of model group(P<0.01~P<0.05).The large and medium doses could greatly increase moisture content in the mice skin(P<0.01~P<0.05). The small dose showed some influence on the above indexes

but the effect was not significant.The hemp seed oil could improve the fur growing condition of the model mice.The dermal thickness and the number of hair follicles of the model mice were considerably increased. The texture of dermal collagen fibers and elastic fibers were obviously improved. Conclusion: The hemp seed oil can exert its function of anti-skin-aging and antioxidation.