Comparison of Different Methods for Extraction of Genomic DNA from

ZHU Tian-tian; JIN Ling; DU Tao; CHEN Hong-gang; ZHANG Yan-hong; WANG Hui-zhen; WANG Yan

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Comparison of Different Methods for Extraction of Genomic DNA from

更新时间：2024-01-04

- Comparison of Different Methods for Extraction of Genomic DNA from
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 11, Pages: 125-128(2012)
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- Published：2012
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ZHU Tian-tian, JIN Ling, DU Tao, et al. Comparison of Different Methods for Extraction of Genomic DNA from [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 125-128.
DOI：

ZHU Tian-tian, JIN Ling, DU Tao, et al. Comparison of Different Methods for Extraction of Genomic DNA from [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 125-128. DOI：

摘要

目的: 比较不同DNA提取方法

选择最适于中麻黄基因组的方法。方法: 采用改良CTAB

SDS法、试剂盒法提取中麻黄基因组DNA;用琼脂糖凝胶电泳法和紫外分光光度法检测所得总DNA的得率和纯度

并进行ISSR-PCR扩增检测。结果: 3种方法均可从新鲜中麻黄中提取出产量较高的基因组DNA

但其中改良CTAB法提取的总DNA纯度高

质量好

扩增产物的电泳条带也较明显;SDS法和试剂盒法提取的总DNA质量差

不适用于下游分子生物学实验。结论: 改良十六烷基三甲基溴化铵(CTAB)法为中麻黄基因组DNA提取的最佳方法

该方法提取的基因组DNA适用于中麻黄基因组PCR扩增和其他分子生物学研究。

Abstract

Objective: By comparing different extraction methods of genomic DNA

the best extraction method of Ephedra intermedia genomic DNA was selected. Method: Improved cetyltrimethyl ammonium bromide(CTAB) method

SDS method

kit method were used for the extraction of genomic DNA from E. intermedia. The yield and purity of total genomic DNA were examined by agarose gel electrophoresis

UV-spectrophotometry and ISSR-PCR. Result: Three extraction methods could extracted the high amount genomic DNA from fresh tissue of E. intermedia. Improved CTAB method could yield highest purity total DNA

and the electrophoretic bands of amplified products were obvious. On the other hand

SDS method and kit method indicated low purity of the yielded DNA and could not be used in downstream application directly. Conclusion: The improved CTAB method is the best of the three compared methods for extracting the genomic DNA from E. intermedia

which is suitable for PCR reaction and other molecular research in E. intermedia genomic DNA.

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