Optimization of Separation and Purification Process for Total Saponins from  with Macroporous Resin

WANG Chang-ling; ZHU Xiang; FU Yan-ling; HU Kai-wen; AN Chao; NI Sheng-lou; HAN Li-wei

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Optimization of Separation and Purification Process for Total Saponins from with Macroporous Resin

更新时间：2024-01-04

- Optimization of Separation and Purification Process for Total Saponins from with Macroporous Resin
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 11, Pages: 28-31(2012)
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- Published：2012
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WANG Chang-ling, ZHU Xiang, FU Yan-ling, et al. Optimization of Separation and Purification Process for Total Saponins from with Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 28-31.
DOI：

WANG Chang-ling, ZHU Xiang, FU Yan-ling, et al. Optimization of Separation and Purification Process for Total Saponins from with Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 28-31. DOI：

摘要

目的: 优选大孔树脂分离纯化土贝母总皂苷的工艺条件。方法: 以吸附量和解吸率为考察指标

通过静态和动态试验比较不同型号大孔树脂对土贝母总皂苷的分离纯化性能

选出最佳树脂并对其纯化工艺进行优选。结果: HPD-750型树脂对土贝母总皂苷有较好的吸附分离性能

最佳工艺条件为树脂径高比为1 :5

上样液质量浓度1.6 g·mL-1

最大上样量为13 mL

1 BV水洗除杂

8 BV 95%乙醇洗脱

吸附和洗脱流速均为1 mL·min-1

收集洗脱液

回收乙醇至干

采用紫外分光光度法检测总皂苷质量分数为63.53%。结论: 该优选工艺简单易行

能较好地分离纯化土贝母总皂苷。

Abstract

Objective: To optimize separation and purification process parameters of total saponins from Bolbostemma paniculatum by macroporous resin. Method: With absorption and desorption rate of total saponins as indexes

separation and purification performance of macroporous resin on total saponins from B. paniculatum was compared by static and dynamic test

optimum resin was selected and its purification technology was optimized. Result: HPD-750 macroporous resin showed the best property for purification of total saponins.its optimal process conditions were:diameter-height ratio 1 : 5

the concentration of sample solution 1.6 g·mL-1

maximum sample volume 13 mL

eluted by 1 BV water and 8 BV 95% ethanol

flow rate of adsorption and elution 1 mL·min-1

eluent were collected

ethanol was recovered

under these conditions

the content of total saponins was 63.53% by UV. Conclusion: This optimized process was simple and feasible to separate and purify total saponins from B. paniculatum effectively.

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Related Author

WEI Dong-qing

CHEN Shao-min

MIAO Jian-wu

DING Jia-xin

CHEN Zhi

TIAN Jing-zhen

HAN Jing

FENG Li-jun

Related Institution

Medical Molecular Biology Laboratory,Luzhou Medical College

Drug and Functional Food Research Center,Luzhou Medical College

College of pharmacy,Beijing University of Chinese Medicine

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

College of Food Science and Biology， Hebei University of Science and Technology

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