Objective: To evaluate accumulation of the volatiles of the different tissues in Magnolia officinalis. Method: The volatiles of leaf buds

root skins

barks

twig skins

leaves

pericarps and seeds of M. officinalis were analyzed using headspace solid phase micro-extraction(HS-SPME) following by capillary gas chromatography coupled to mass spectrometry (GC-MS). Result: The volatiles were α-linalool 0.21%-4.92%

α-caryophyllene 7.48%-38.71%

β-caryophyllene 1.53%-14.43%

eudesma-4(14)

11-diene 0.15%-10.02%

β-chamigrene 0.56%-10.68%

caryophyllene oxide 1.68%-36.01%

α-eudesmol 1.56%-33.79% and β-eudesmol 0.19%-28.89%. The yields of essential oils in the different tissues were 0.12%-0.96%. Conclusion: The essential oil

the volatiles composition and their accumulation content of leaf buds

root skins

barks

twig skins

leaves

pericarps and seeds of M. officinalis were significantly different.