Objective: To study the mobilization of peripheral blood stem cell in mice by compound fufangteng mixture. Method: Sixty mice were divided into four groups at random (n=15 each).Control group was treated with normal saline; fufangteng mixture group was treated with fufangteng mixture

granulocyte colony-stimulating factor(G-CSF) group was treated with G-SCF

and Fufangteng mixture combined G-CSF group group was treated withy G-SCF and Fufangteng mixture. The number of peripheral white blood cell(WBC) and mononuclear cell(MNC) was counted after 6 days

the number of colony-unit-mix(CFU-Mix) colony was cultured by methylcellulose micro-culture

and number and percentage of stem cell antigen 1(Sca-1) cells were counted by flow cytometry. Result: The number of peripheral WBC and MNC was higher than pre-treatment

exceptfor control group. The number of WBC was (7.5±0.9)

(9.0±0.6)

(16.1±1.4)

(19.8±0.4)×106/L(P<0.05) respectively

while the number of MNC was (4.0±0.7)

(6.6±0.3)

(10.5±0.9)

(12.6±1.4)×106/L (P<0.05) respectively. The yields of CFU-Mix were higher than control group. Meanwhile

the percentage of Sca-1+ in peripheral blood stem cells was higher than control group

and the percentage was 0.79%

1.15%

1.72%

respectively. Conclusion: Compound Fufangteng mixture has an obvious mobilizing effete on murine peripheral bloods tem cells. The effect of Fufangteng mixture combined with G-CSF is better than single usage of compound Fufangteng mixture or G-CSF.