Optimization of Extraction Technology for Total Flavonoids from  by Multi-index Comprehensive Evaluation Method

WANG Xing; SHI Yan-qiong; QIN Hong-en; GUO Dong-yan

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Optimization of Extraction Technology for Total Flavonoids from by Multi-index Comprehensive Evaluation Method

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- Optimization of Extraction Technology for Total Flavonoids from by Multi-index Comprehensive Evaluation Method
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 13, Pages: 46-49(2012)
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- Published：2012
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WANG Xing, SHI Yan-qiong, QIN Hong-en, et al. Optimization of Extraction Technology for Total Flavonoids from by Multi-index Comprehensive Evaluation Method[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(13): 46-49.
DOI：

WANG Xing, SHI Yan-qiong, QIN Hong-en, et al. Optimization of Extraction Technology for Total Flavonoids from by Multi-index Comprehensive Evaluation Method[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(13): 46-49. DOI：

摘要

目的: 优选湖北海棠中总黄酮的提取工艺。方法: 以总黄酮及根皮苷含量为指标

采用单因素试验法筛选提取方法和提取次数;以浸膏得率

总黄酮及根皮苷含量为综合评价指标

对主要影响因素乙醇体积分数、溶剂用量、提取时间进行正交试验优选。结果: 最佳提取工艺为8倍量70%乙醇回流提取3次

每次1 h。结论: 优选工艺稳定可行

可作为湖北海棠中总黄酮的提取工艺。

Abstract

Objective: To optimize extraction technology of total flavonoids from Malus hupehensis. Method: Single factor test was made to select extraction method and extraction times with the content of total flavonoids and phloridzin as indexes;With the content of extract yield

total flavonoids and phloridzin as comprehensive evaluation index

main factors were optimized by orthogonal test which including the concentration of ethanol

the amount of solvent and extraction time. Result: Optimum extraction technology was:refluxing extracted 3 times with 8 folds the amount of 70% ethanol

1 h each time. Conclusion: Optimized process was stable and feasible

and it could be extraction process of total flavonoids from M. hupehensis.

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Related Author

LI Ya

LI Kai-yan

PEI Dan

ZHAO Ming

OUYANG Zhen

CAO Xu

YANG Xi-hui

LIU Yue-qin

Related Institution

Guilin Medical University

Affiliated Hospital of Guilin Medical College

Zhejiang Chinese Medical University

Zhejiang Province Hospital of Traditionl Chinese Medicine

School of Pharmacy,Jiangsu University

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