Objective: To establish a method for measuring the content of Ginsenoside Rb1 in Shensong Yangxin capsules by HPLC

in order to provide theoretical basis for the quality standards of Shensong Yangxin capsules. Method: The Ginsenoside Rb1 from Shensong Yangxin capsules was extracted by methanol reflux extraction 2 h

and then extracted by water-saturated n-butanol with

ammonia solution washing. The content of Ginsenoside Rb1 in Shensong Yangxin capsules was measured by HPLC

the instrument and chromatographic conditions was Agileni1100 high-performance liquid chromatograph

waters XTERRA MS C18(4.6 mm×250 mm

5 μm)

column temperature: room temperature; mobile phase: acetonitrile-water (53∶47); flow rate 1.0 mL·min-1; detection wavelength 203 nm; injection volume 40 μL. Result: The Ginsenoside Rb1 within the range of 5.0-200.0 mg·L-1 was linear with the peak area

r 0.998 9; the average recovery was 100.31%

RSD 1.98%. Conclusion: The established method has strong specificity

high accuracy and good reproducibility

it can be used as quality control of Shensong Yangxin capsules.