Optimization of Extraction Process for  and  from Baijin Fuan Granule

CAI Mei-chao

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Optimization of Extraction Process for and from Baijin Fuan Granule

更新时间：2024-01-04

- Optimization of Extraction Process for and from Baijin Fuan Granule
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 13, Pages: 53-55(2012)
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- Published：2012
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CAI Mei-chao. Optimization of Extraction Process for and from Baijin Fuan Granule[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(13): 53-55.
DOI：

CAI Mei-chao. Optimization of Extraction Process for and from Baijin Fuan Granule[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(13): 53-55. DOI：

摘要

目的: 优选白金妇安颗粒中白芍和柴胡的提取工艺。方法: 以HPLC测定芍药苷提取量

分光光度法测定的柴胡总皂苷提取量

采用单因素试验法优选提取溶剂

正交试验法对白金妇安颗粒提取工艺条件进行优选。结果: 以70%乙醇为溶剂时芍药苷提取量最多

乙醇体积分数>50%时

柴胡总皂苷提取量基本不变;优选提取工艺白芍、柴胡粉碎成粗粉

分别加8

6倍量70%乙醇回流提取2次

每次2 h。结论: 优选工艺合理

有效成分提取率高。

Abstract

Objective: To optimize extraction process of Paeonia lactiflora and Bupleurum chinense from Baijin Fuan granule. Method: With extraction volume of paeoniflorin and total saponins from B. chinense as indexes which were determined by HPLC

extraction solvent was optimized by single-factor test;With the content of paeoniflorin and total saponins from B. chinense as indexes

extraction process of Baijin Fuan granule was optimized by orthogonal test. Result: Extraction volume of paeoniflorin was the most with 70% ethanol as solvent

but when the concentration of ethanol was more than 50%

total saponins from B. chinense was basically unchanged;Optimized extraction technology was P. lactiflora and B. chinense were crushed into coarse powder

refluxing extracted 2 times with 8

6 times the amount of 70% ethanol

respectively.2 h per time. Conclusion: Optimized process was reasonable with high yield of active ingredients.

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LI Ying

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HU Longxiao

YUAN Yue

CHEN Xiaoxiao

SHI Yali

REN Junguo

Related Institution

Natural History Museum of China

Beijing Key Laboratory of Chinese Materia Pharmacology，Institute of Basic Medical Sciences， Xiyuan Hospital，China Academy of Chinese Medical Sciences

Beijing University of Chinese Medicine

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

School of Chinese Materia Medica， Beijing University of Chinese Medicine

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