Determination of Bergenin in  by HPLC

LIU Yuan; SONG Zhi-zhao; WEN Zhi-yun

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Determination of Bergenin in by HPLC

更新时间：2024-01-04

- Determination of Bergenin in by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 14, Pages: 79-80(2012)
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- Published：2012
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LIU Yuan, SONG Zhi-zhao, WEN Zhi-yun. Determination of Bergenin in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(14): 79-80.
DOI：

LIU Yuan, SONG Zhi-zhao, WEN Zhi-yun. Determination of Bergenin in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(14): 79-80. DOI：

摘要

目的: 建立翼茎白粉藤中岩白菜素的含量测定方法。方法: 采用高效液相色谱法

以Appollo-C18柱(4.6 mm×250 mm

5 μm)为分离柱

以甲醇-水(22∶78)为流动相

286 nm为检测波长

柱温为室温。结果: 岩白菜素在10.6~169.6 mg·L-1

峰面积与其质量浓度呈良好线性关系(r=0.999 2)

平均回收率为100.5%

RSD 1.53%(n=6)。结论: 该方法简便、准确、重复性好

可作为该药材含量测定方法。

Abstract

Objective: To develop an HPLC method for detecting bergenin in Cissus pteroclada. Method: An Appollo-C18 column(4.6 mm×250 mm

5 μm)was used. The mobile phase consisted of methanol-water (22∶78). The detection wavelength was 286 nm and the column temperature was room temperature. Result: Bergenin showed a good linear relationship at the range of 10.6 to 169.6 mg·L-1

r=0.999 2.The recovery and RSD were 100.5% and 1.53%(n=6)

respectively. Conclusion: This method was proved to be simple

accurate and repeatable to determine the content of bergenin in C. pteroclada.

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Related Institution

Guiyang College of Traditional Chinese Medicine

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Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

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Jilin Huakang Pharmaceutical Co. Ltd.

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