Effect of Baixiangdan Capsule on Expression of GABAR and Adenylate Cyclase of Frontal Cortex in Rats with Anxiety Emotion
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Effect of Baixiangdan Capsule on Expression of GABAR and Adenylate Cyclase of Frontal Cortex in Rats with Anxiety Emotion
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 13, Pages: 153-156(2012)
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Published:2012
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CAI Hong-xin, XU Li-li, YIN Hui-min, et al. Effect of Baixiangdan Capsule on Expression of GABAR and Adenylate Cyclase of Frontal Cortex in Rats with Anxiety Emotion[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(13): 153-156.
DOI:
CAI Hong-xin, XU Li-li, YIN Hui-min, et al. Effect of Baixiangdan Capsule on Expression of GABAR and Adenylate Cyclase of Frontal Cortex in Rats with Anxiety Emotion[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(13): 153-156.DOI:
Effect of Baixiangdan Capsule on Expression of GABAR and Adenylate Cyclase of Frontal Cortex in Rats with Anxiety Emotion
Objective: To detect the expression of γ-aminobutyric acid B receptor (GABABR)and adenylate cyclase (AC) of forbral cortex in rats with anxiety emotion and to explore the antianxiety mechanism of baixiangdan capsule. Method: Paeoniflorin
cyperolone and paeonolum were main ingredients of baixiangdan capsule. Wistar rats were divided into normal group
model group
baixiangdan capsule group(200 mg·kg-1) and baclofen group(8 mg·kg-1). The rats were administrated with relevant drugs intragastrically once a day for 7 days. The anxiety emotion rat models were established by a social isolation plus resident intruder stress. The rats were evaluated by sucrose intake
the open field and elevated plus-maze tests. The expression of GABABR1
GABABR2 and AC in frontal cortex were detected by the immunoflourescence. Result: Compared with normal group
the model group had the lower sucrose intake
the higher total score of open field tests
the lower open arm entry percent(OE%) and the lower open arm time percent (OT%) of elevated plus-maze tests and the lower expression of GABABR1
GABABR2 and AC in frontal cortex. Compared with model group
baixiangdan capsule and baclofen decreased the total score of open field tests
increased the OE%and OT%of elevated plus-maze tests and the expression of GABABR1
GABABR2 and AC in frontal cortex. Conclusion: The lower expression of GABABR1
GABABR2 and AC in frontal cortex may have close relationship with anxiety emotion. Increasing the expression and the function of GABABR in frontal cortex maybe one of the mechanisms of the antianxiety effect by Baixiangdan capsule.
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