The Evaluation of Genotoxicity on Radix Millettiae Speciosae by Singe Cell Gel Electrophoresis

CHEN Xiao-bai; WANG Xiao-ping; HUANG Xiao-ting

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The Evaluation of Genotoxicity on Radix Millettiae Speciosae by Singe Cell Gel Electrophoresis

更新时间：2024-01-04

- The Evaluation of Genotoxicity on Radix Millettiae Speciosae by Singe Cell Gel Electrophoresis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 12, Pages: 222-225(2012)
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- Published：2012
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CHEN Xiao-bai, WANG Xiao-ping, HUANG Xiao-ting. The Evaluation of Genotoxicity on Radix Millettiae Speciosae by Singe Cell Gel Electrophoresis[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(12): 222-225.
DOI：

CHEN Xiao-bai, WANG Xiao-ping, HUANG Xiao-ting. The Evaluation of Genotoxicity on Radix Millettiae Speciosae by Singe Cell Gel Electrophoresis[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(12): 222-225. DOI：

摘要

目的: 应用单细胞凝胶电泳技术评价牛大力的遗传毒性。方法: 40只小鼠随机分成5组:阳性对照组(环磷酰胺组)、阴性对照组(生理盐水组)、牛大力高、中、低(20

5 g·kg-1)剂量组。牛大力各剂量组用牛大力煎煮液ig

阴性对照组、阳性对照组ig等量生理盐水

每天1次

连续10 d

阳性对照组在最后2 d ip环磷酰胺(0.08 g·kg-1)

每天1次

在最后1次给药6 h后

处死各组小鼠

取肺、肾、肝、睾丸细胞

利用单细胞凝胶电泳技术观察牛大力对小鼠肺、肾、肝、睾丸细胞DNA的影响。结果: 牛大力各剂量组对小鼠肺、肾、肝、睾丸细胞的尾部DNA百分率(Tail DNA％)和尾矩(Tail Moment)的影响明显低于阳性对照组

差异有统计学意义(P<0.01)

牛大力高剂量组肾细胞Tail DNA%与阴性对照组比明显降低

统计学上有显著差异(P<0.05)

其他各剂量组小鼠细胞tail DNA%和tail moment与阴性对照组比

差异无统计学意义。结论: 牛大力对小鼠肺、肾、肝、睾丸细胞DNA未观察到明显损伤。

Abstract

Objective: To evaluate genotoxicity of Radix Millettiae Speciosae by single cell gel electrophoresis. Method: Forty mice were divided into five groups randomly: negative control group

positive control group

and Radix Millettiae Speciosae group with high(20 g·kg-1)

middle (10 g·kg-1)or low(5 g·kg-1) doses. Radix Millettiae Speciosae groups were given with relative drugs

Negative control group and positive control group were given with normal saline by gavage once a day for 10 days

while the last two days

positive control group was injected with cyclophosphamide(0.08 g·kg-1)once a day. In the eleventh day

the mice were sacreficed

DNA damage of liver

kidney

lung

testicular cells was detected by single cell gel electrophoresis. Result: Compared with positive control group

tail DNA% and tail moment of liver

kidney

lung

testicular cells in Radix Millettiae Speciosae group had a significant lowereduction (P<0.01).Compared with the negative control group

tail DNA%and tail moment of kidney cells in the Millettiae Speciosae group with high dose showed a significant reduction (P<0.05)

tail moment and tail DNA% of ther cells in other dose group

no statistically significant difference was found. Conclusion: Radix Millettia Speciosa has no damage to mouse cells DNA.

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