Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 11, Pages: 116-117(2012)
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Published:2012
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WANG Wen-lai, GAO Zhi-hong, YU Zhi-min, et al. Determination of Baicalein in Qinloukeli by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 116-117.
DOI:
WANG Wen-lai, GAO Zhi-hong, YU Zhi-min, et al. Determination of Baicalein in Qinloukeli by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 116-117.DOI:
Objective: Determination of baicalein in Qinloukeli by HPLC. Method: The separation was performed on Alltech C18(4.6 mm×250 mm
5 μm) at 25 ℃. The mobile phase was composed of methanol-water-phosphorous acid solution (47 : 53 : 0.2) with a flow rate of 1.0 mL·min-1. The detective wavelength is at 280 nm. Result: Hesperidin was linear within the range of 0.057 2-0.629 2 μg(r=0.999 9). The average recovery for hesperidin was 99%
and RSD was 1.23%. Conclusion: The method was selective
accurate and simple
and suitable for controlling the quality of Qinloukeli.