Objective: To study the effects of Paning decoction on behavior and oxidative stress reaction in rats with Parkinson's disease(PD). Method: The PD model were established through stereotoxic microinjection of 6-OHDA. Fifty SD rats were randomly divided into five groups: model group

positive group(madopar 0.125 g·kg-1)and the high

middle and low dose of Paning decoction groups(84

42

21 g·kg-1). In addition

a normal group and a sham operated group were established. The behavior (number of rotation) was observed before and after treatment. Moreover

the contents of reactive oxygen species(ROS)

malondialdehyde(MDA) and glutathione(GSH)

the activity of glutathione peroxidase(GSH-Px) and superoxidedismutase(SOD) in themed brain nigro-striatum were detected. Result: Compared with the model group

the number of rotation was 237.26±19.23

246.71±37.06 and 278.81±20.09 (in high

middle and low dosage groups

respectevly). the content of ROS were (231.02±10.11)

(235.87±12.76)

(247.93±11.30) U·mg-1

and the content of MDA were (4.56±1.25)

(4.70±1.17)

(4.63±1.06) nmol·mg-1

respectevly. The content of ROS and MDA were decreased significantly. On the other hand

the content of GSH were (6.20±0.81)

(6.24±0.76)

(6.18±0.90) mg·g-1

the activities of GSH-Px were (2.68±0.27)

(2.59±0.34)

(2.31±0.22) nU·g-1

SOD were (188.40±5.86)

(170.87±9.80)

(165.62±6.54) nU·mg-1

respectively. The content of GSH and the activity of GSH-Px

SOD were increased significantly. All the experimental data had statistical significance (P<0.05). Conclusion: Paning decoction can improve the behavior in PD rats

furthermore can reduce the injury of oxidative stress.