Objective: To establish an RP-HPLC method for determination of bacalin and berberin hydrochloride in Sanhuang tablets. Method: The separation was performed on an Accurasil C18(4.6 mm×250 mm

5 μm) column eluted with a mobile phase consisted of acetonitrile-0.2% phosphoric acid solution (25:75) at a flow rate of 1.0 mL·min-1. The column temperature was set at 35℃

and the detection wavelength was set at 270 nm. Result: The linear ranges of bacalin and berberin hydrochloride were 0.043 6-0.436 μg(r=0.9999) and 0.018 5-0.185 μg(r=0.9999)

the average recoveries of bacalin and berberin hydrochloride were 105.0% and 103.0%

and RSD were 1.28% and 1.80% respectively. Conclusion: The method was simple

feasible

and reproducible

and it can be used for the quality control of Sanhuang Tablet.