Antioxidation of Different Extracts from

CHEN Han; LI Jin; LI Xiang; CHEN Jian-wei; XUE Ping

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Antioxidation of Different Extracts from

更新时间：2024-09-23

- Antioxidation of Different Extracts from
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 9, Pages: 184-186(2012)
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- Published：2012
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CHEN Han, LI Jin, LI Xiang, et al. Antioxidation of Different Extracts from [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 184-186.
DOI：

CHEN Han, LI Jin, LI Xiang, et al. Antioxidation of Different Extracts from [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 184-186. DOI：

摘要

目的: 考察板蓝根不同提取部位的体外抗氧化活性。方法: 用大孔树脂和阴、阳离子交换树脂制备板蓝根3个有效部位:总木脂素、总有机酸、总生物碱部位。结合酶标仪

通过清除1

1-二苯基苦基苯肼(DPPH)自由基和Fe3+还原能力(FRAP)测定板蓝根提取物的体外抗氧化作用。结果: 板蓝根颗粒组及各个提取物组均有不同程度的体外抗氧化效果。其中以总木脂素抗氧化效果最佳(DPPH IC50 160.6 mg·L-1; FRAP 0.75 mmol·g-1);其次为板蓝根颗粒组(DPPH IC50 595.97 mg·L-1; FRAP 0.12 mmol·g-1);总有机酸

总生物碱部位效果较弱。结论: 测定了板蓝根中不同有效部位的抗氧化活性

初步确定其抗氧化相关活性成分主要存在于木脂素部位。

Abstract

Objective: Antioxidation of different extracts from Isatidis indigotica were studied in vitro to supply the separating base of antioxidative components. Method: The extracts were prepared by macroporous resin and anion/cation exchange resin. Their antioxidant activities were assessed by two complementary test systems

namely 1

1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and ferric reducing antioxidant power (FRAP) assay. Result: The total lignin displayed strong activity in all test systems. (DPPH IC50 160.6 mg·L-1; FRAP 0.78 mmol·g-1) Banlangen Keli (DPPH IC50 595.97 mg·L-1; FRAP 0.12 mmol·g-1) showed relatively lower activity. Total organic acids and total alkaloid have a little activity. Conclusion: Antioxidative components could be justified existing in the total lignin initially.

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