Objective: To establish a test model for phototoxic chemicals with the method of chemiluminescence and assess the phototoxicity of different components of Radix Echinopsis. Method: Nicotinamide adenine dinucleotide reduced(NADH) and photosensitive agents was irradiated with vitraviolet A(UVA)

then luminol was added to the test samples

and chemiluminescent value was counted and calculated by an ultra weak chemiluminescence analyzer. Result: Chemiluminescent values of the α-terthienyl concerned with the increase of UVA dosage

and there was a dose-effect relationship in the concentration range of 1.0×10-9-2.5×10-2 g·L-1. Conclusion: This method is simple

accurate and sensitive. It can be used for pre-assessment of phototoxicity.