Simultaneous Determination of Three Active Ingredients in Wujia Shenghua Capsule by HPLC

GENG Fang; ZHU Kui-yuan; WANG Fa-shan; ZOU Tao-bo; LI Tie-nan; ZHANG Ning

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Simultaneous Determination of Three Active Ingredients in Wujia Shenghua Capsule by HPLC

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- Simultaneous Determination of Three Active Ingredients in Wujia Shenghua Capsule by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 9, Pages: 109-111(2012)
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- Published：2012
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GENG Fang, ZHU Kui-yuan, WANG Fa-shan, et al. Simultaneous Determination of Three Active Ingredients in Wujia Shenghua Capsule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 109-111.
DOI：

GENG Fang, ZHU Kui-yuan, WANG Fa-shan, et al. Simultaneous Determination of Three Active Ingredients in Wujia Shenghua Capsule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 109-111. DOI：

摘要

目的: 建立同时测定五加生化胶囊中3种有效成分阿魏酸、绿原酸和异秦皮啶的含量分析方法。方法: 采用高效液相色谱法

ANGLA Venusil XBP-C18 (4.6 mm×250 mm

5 μm)色谱柱

流动相甲醇-0.1%磷酸水梯度洗脱

检测波长323

327

343 nm

流速1.0 mL·min-1

柱温25 ℃

进样量20 μL。结果: 在上述色谱条件下

绿原酸、阿魏酸、异秦皮啶之间有良好的分离度

绿原酸(λ=327 nm)线性范围18.14~108.84 mg·L-1(r=0.999 8);异秦皮啶(λ=343 nm)线性范围5.10~30.60 mg·L-1(r=0.999 5);阿魏酸(λ=323 nm)线性范围7.34~44.04 mg·L-1(r=0.999 4)

平均回收率分别为(97.92±1.751)%

(98.82±1.494)%

(104.0±1.355)%。结论: 该方法简便、准确

重复性好

为五加生化胶囊的质量控制提供可借鉴的分析方法。

Abstract

Objective: To establish a HPLC method for simultaneous determination of chlorogenic acid

ferulic acid and isofraxidine in Wujia Shenghua capsules. Method : The chromatographic separation was performed on a Welch Materials XBP-C18 column (4.6 mm×250 mm

5 μm)

the column temperature was maintained at 25 ℃. The UV detection wavelengths were at 327

323

343 nm. A gradient elution of methanol- 0.1% phosphoric acid aqueous solution was adopted at the flow rate of 1 mL·min-1. The injection volume was 20 μL. Result: The good resolution can be achieved among chlorogenic acid

ferulic acid and isofraxidine. Chlorogenic acid

ferulic acid and isofraxidine were linear in the range of 18.15-108.90 mg·L-1 (r=0.999 8)

5.10-30.60 mg·L-1 (r=0.999 5) and 7.35-44.10 g·L-1 (r=0.999 4)

respectively. The average recoveries (n=6) were between 95% and 105%. Conclusion: The method is accurate

easy and reproducible

which can be applied to quality control of Wujia Shenghua capsule.

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Related Author

GENG Fang1

ZHU Kui-yuan2

WANG Fa-shan3

ZOU Tao-bo2

LI Tie-nan2

ZHANG Ning2

LI Xiong

YANG Liu

Related Institution

Harbin Normal University

Jiamusi College,Heilongjiang University of Chinese Medicine

DUODUO Pharmaceutical Co. LTD.

Second Affiliated Hospital,Guangzhou University of Traditional Chinese Medicine

Guangdong Pharmaceutical University

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