Objective: To determine the content of the hesperidin in six different kinds of pericarpium citri reticulatae and gained a method to estimate and control the quality of medical material and effectively. Method: The HPLC was adopted to do this examination

the separat on was performed on a Baseline C18 column (4.60 mm×250 mm

5 μm)

methanol-water (1% acetic acid solution)(50∶50) was used as the mobile phase with isocratic elution

the detective wavelength was at 283 nm

the flow rate was 1.0 mL·min-1

the column temperature was room temperature and the injecti on volume was 20 μL. Result: The hesperidin showed a good linearity in the range of 0.002-0.040 g·L-1(r=0.999 9)

the average recovery was 102.3% (n=9)

RSD 1.6%. The contents of hesperidin in peels of citrus was varied from different species. Conclusion: The method is simple and available

which can be used to determine and estimate hesperidin in different kinds of pericarpium citri reticulatae.