Effects of Dahuang Fuzi Decoction on the Functions of Peritoneal Macrophages in BALB/c Mice

WU Li; LIU Xiao; CAI Hao; LV Gao-hong; GUO Hui; ZHANG Yin; CAI Bao-chang

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Effects of Dahuang Fuzi Decoction on the Functions of Peritoneal Macrophages in BALB/c Mice

更新时间：2024-09-23

- Effects of Dahuang Fuzi Decoction on the Functions of Peritoneal Macrophages in BALB/c Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 9, Pages: 176-179(2012)
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- Published：2012
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WU Li, LIU Xiao, CAI Hao, et al. Effects of Dahuang Fuzi Decoction on the Functions of Peritoneal Macrophages in BALB/c Mice[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 176-179.
DOI：

WU Li, LIU Xiao, CAI Hao, et al. Effects of Dahuang Fuzi Decoction on the Functions of Peritoneal Macrophages in BALB/c Mice[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 176-179. DOI：

摘要

目的: 研究大黄附子汤对BALB/c小鼠腹腔巨噬细胞功能的影响。方法: 制备小鼠腹腔巨噬细胞

纯化后以5×105/mL细胞接种于96孔培养板或6孔板中

分为:细胞对照组、脂多糖(lipopolysaccharide

LPS)模型组(10 mg·L-1)、大黄附子汤组(终质量浓度为25

100

200

400

800 mg·L-1)

在细胞贴壁过夜后

正常组给予不含血清的培养液

其余各组给予LPS(10 mg·L-1)

大黄附子汤组同时给予不同浓度药物

48 h后测细胞存活率

ELISA法测肿瘤坏死因子-α (TNF-α)

白介素-6(IL-6)水平

黄嘌呤氧化酶法(羟氨法)测细胞上清液中超氧化物歧化酶(SOD)活力

硫代巴比妥酸法测丙二醛(MDA)含量

Griess法测一氧化氮(NO)水平。结果: 在25~400 mg·L-1内

大黄附子汤对正常细胞代谢MTT活力无显著影响;剂量达50 mg·L-1即能抑制LPS诱导NO的生成(P＜0.01)

100 mg·L-1时能降低MDA含量(P＜0.01)

增强SOD酶活力(P＜0.05)

并能显著抑制LPS诱导TNF-α

IL-6等细胞因子的合成(P＜0.01)。结论: 大黄附子汤能有效调节BALB/c小鼠腹腔巨噬细胞免疫及抗氧化功能

改善LPS对BALB/c小鼠腹腔巨噬细胞的诱导作用。

Abstract

Objective : To investigate the effects of Dahuang Fuzi decoction on the function of peritoneal macrophages inmice. Method : Macrophages were seeded onto glass cover slips (5×105 cells/mL).Different concentrations of Dahuang Fuzi decoction(25

100

200

400

800 mg·L-1) were added to the normal or LPS-activited peritoneal macrophages. Metabolic activity of peritoneal macrophages was measured by MTT assay

nitric oxide was detected by Griess reagent

malondiadehyde(MDA) content was detected by hydroxylamine method

superoxide dismutase(SOD) activities was assayed by thiobarbituric acid method

concentrations of tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) in supernatants were determined by using quantitative sandwich enzymelinked immunosorbent assay(ELISA)kits. Result : Dahuang Fuzi decoction had no obvious effect on the metabolic activity of peritoneal macrophages

but could markedly inhibit the secretion of NO

MDA

TNF-α and IL-6(P＜0.01)

furthermore

Dahuang Fuzi decoction could improved the production of SOD(P＜0.05). Conclusion : Dahuang Fuzi decoction can promote the immune and antioxidant capacity of peritoneal macrophagesin BALB/c mice.

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Related Author

WU Li1

LIU Xiao1

CAI Hao1

LV Gao-hong1

GUO Hui1

ZHANG Yin1

CAI Bao-chang1

SHEN Zhixiang

Related Institution

College of Pharmacy,Nanjing University of Chinese Medicine

Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing,Nanjing University of Chinese Medicine

Key Laboratory of State Administration of Traditional Chinese Medicine for Standardization of Chinese Medicine Processing, Nanjing University of ChineseMedicine

Affiliated Hospital of Nanjing University of Chinese Medicine

Provincial Key Laboratory of Entomology Biopharmaceutical R&D，Dali

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