Objective: To established an RP-HPLC method for determination of quercetin in Pyracantha fortuneana and to compare quercetin contents in different medicinal parts. Method: 90%methanol-25% hydrochloride aqeous solution (4 : 1) was used to extract quercetin and Hypersil ODS (4.6 mm×250 mm

5 μm) column was used to analyze quercetin content with methanol-0.1% phosphoric acid aqueous solution as mobile phases. Flow rate was 1.0 mL·min-1. Detection wavelength was set at 360 nm. Result: The standard curve of quercetin was linear in the range of 0.048 5-1.552 0 μg.The average recovery of quercetin was 98.44%. Conclusion: Quercetin contents in different medicinal parts of P. fortuneana was different.