Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 11, Pages: 94-97(2012)
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Published:2012
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SHEN Hai-yan, Gong Xiao-jian, LI Wen-min, et al. Determination of Total Saponins in [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 94-97.
DOI:
SHEN Hai-yan, Gong Xiao-jian, LI Wen-min, et al. Determination of Total Saponins in [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 94-97.DOI:
Objective: The quantitative method was established of total saponins in Periploca forrestii and the content of the general saponins of Periploca forrestii from different places was determined. Method: Ursolic acid was selected as control article.Coloration was used with 5% vanillin and perchloric acid.The content of total saponins in P. forrestii was determined at 550 nm by UV. Result: The calibration curve was linear over the range of 9-55 mg·L-1 with the correlation of 0.999 2
and the linear regression equation was A=0.011 9 C+0.097 2.The average recovery of ursolic acid was 97.16% with RSD of 1.3%(n=6). The contents of the total saponins in P. forrestii from various habitats was quite different. Conclusion: The method is accurate
simple
sensitive and reliable. So it can be used to determine the content of total saponins in P. forrestii.
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