Total Flavonoids Determination of  by Ultraviolet Spectrophotometry

XU Ling-yuan; XIE Ji-zhao; XIE Yun-feng; QIU Li; QIN Qing; HUANG Gui-kun; XIE Ting

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Total Flavonoids Determination of by Ultraviolet Spectrophotometry

更新时间：2024-01-04

- Total Flavonoids Determination of by Ultraviolet Spectrophotometry
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 12, Pages: 88-90(2012)
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- Published：2012
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XU Ling-yuan, XIE Ji-zhao, XIE Yun-feng, et al. Total Flavonoids Determination of by Ultraviolet Spectrophotometry[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(12): 88-90.
DOI：

XU Ling-yuan, XIE Ji-zhao, XIE Yun-feng, et al. Total Flavonoids Determination of by Ultraviolet Spectrophotometry[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(12): 88-90. DOI：

摘要

目的: 建立一种青天葵药材中总黄酮含量测定的方法

并考察14批青天葵药材中总黄酮的含量。方法: 以芦丁为对照品

AlCl3-HAc-NaAc(pH 5.5)为显色体系

采用紫外分光光度法于最大吸收波长405 nm处测定。结果: 芦丁在7.28~30.80 mg·L-1线性关系良好(r=1.000)

总黄酮平均回收率为100.73%

RSD 1.92%。14批青天葵药材中

总黄酮含量范围为1.25%~3.12%。结论: 该方法操作简便、快速、准确、稳定可靠

可用于青天葵药材中总黄酮的含量测定。

Abstract

Objective: A method for the quality control of Nervilia fordii was developed

and the quality of 14 batches of N. fordii purchased from different markets was investigated. Method: Rutin and AlCl3-HAc-NaAc(pH 5.5)system were employed as reference substance and chromogenic reagent

respectively

the total flavonoids of N. fordii were measured by UV spectrophotometry at the 405 nm. Result: The linear relationship of rutin was in the range of 0.007 28-0.030 8 g·L-1(r=1.000). The average recovery was 99.78％ (RSD 1.94％). The range of the amount of the total flavonoids of 14 batches of N. fordii was 1.25%-3.12%. Conclusion: This method was proved to be simple

quick

accurated

stable and reliable

and it could be used to determine the total flavonoids of N. fordii.

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Related Author

ZHANG Yan1

JIN Xiang-qun1

SHI Ying2

LIU Chang-qing

XIE You-liang

LAI Xiao-ping

Mammat Nurahmat

Tursunjan Abduaini

Related Institution

Pharmacy Technology Center，Tsinghua University

Center of Pharmacy R&D，XinJiang Huachun Bio-Pharmaceutical Co. Ltd.

School of Life Sciences， Tsinghua University

Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences and Peking Union Medical College

School of Pharmacy， Henan University of Chinese Medicine

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