Objective: To observe gene and protein expression of TRPV5 in bone tissue of rats with dexamethasone induced osteoporosis

and to explain pathogenesis of glucocorticoid-induced osteoporosis(GIO)

meawhile to compare the effects of herbs with functions of nourishing-kidney

strengthening-spleen and promoting-blood circulation. Method: One hundrad and thirty two Wistar rats were randomly divided into normal group

model group

nourishing-kidney herb group(1.139 g·kg-1·d-1)

strengthening-spleen herb group(0.945 g·kg-1·d-1)

activating-blood herb group(0.504 g·kg-1·d-1) and Gushukang group(2.1 g·kg-1·d-1). The rat osteoporosis models was replicate byintramuscular injection of dexamethasone (2.5 mg·kg-1)twice a week

lasted for 9 weeks. The BMD of femur in vitro was detected to determine the bone metabolism marker in serum by biochemical process. The mRNA and protein expression of TRPV5 in bone tissue was detected by real-time quantified PCR and Western blot. Result: The BMD of model group decreased obviously(P<0.01)

the content of TRAP in serum increased evidently(P<0.01)

mRNA and protein expression of TRPV5 in bone tissue of model group rats increased. In nourishing-kidney herb group

the BMD up-regulated(P<0.01)

TRAP down-regulated(P<0.01)

and the expression of TRPV5 down-regulated evidently compared with the model group. Conclusion: Nourishing-kidney herbs can inhibit bone resorption of osteoclast through down-regulating mRNA and protein expression of TRPV5 in bone tissue

so have the effect of anti-osteoporosis

which is superior to strengthening-spleen and promoting-blood circulation herbs.