Objective: To establish the method of fingerprint analysis on breviscapine in dispersive tablets and breviscapine material. Method: HPLC with Agilent TC-C18(4.6 mm×250 mm

5 μm) was used

acetonitrile-0.1 mol·L-1 ammonium acetate solution(add phosphoric acid adjust pH to 3.0)

gradient elution as a mobile phase and detection wavelength at 335 nm

flow rate was 0.8 mL·min-1

and column temperature was 35 ℃. Result: The samples quality was assessed by Similarity Evaluation System for Chromatographic Fingerprint of TCM(2.0 edition). The similarity was more than 0.99.Good precision

stability and repetition were showed. Conclusion: The method can be used in the quality control of breviscapine in dispersive tablets and breviscapine material.