Objective: To observe the effects of Danggui Shaoyao San(DSS) on experimental myocardial ischemia in mice. Method: One hundred healthy male Kunming mice were divided into five groups at random:blank control group

model group

DSS low-dose group (raw herb 30 g·kg-1·d-1)

DSS high-dose group (60 g·kg-1·d-1) and positive control group (Fufang Danshen Diwan 0.174 g·kg-1·d-1). Each group was fed with according medicine from the first day to the fifth day. On the fifth day injections of Pit (30 U·kg-1) was given to every group intraperitoneally except the blank control group to which injections of saline were given intraperitoneally. The myocardial ischemia model was duplicated by pit. The electrocardiogram at the tenth minute before the Pit injection and the 5

15

25 min after the Pit injection was recorded. Thirty minutes after the Pit injections the mice were sacrificed

the blood was obtained and the contents of NO were checked up. The tip of myocardium was made into pathological specimen to observe the activities of eNOS by immunohistochemitry method. The rest myocardium was made into tissue homogenate with saline for checking up the activity of SOD and the content of MDA. Result: Compared with the model group

the heart rates in the DSS high-dose and the low-dose groups were recovered significantly (P<0.01 or P<0.05). The contents of serum NO in the DSS high-dose group

the low-dose group were increased significantly (P<0.01). The activities of eNOS in the DSS high-dose group and the low-dose group were enhanced. The activities of SOD in the DSS high-dose group and the low-dose group were increased significantly (P<0.01)

while the contents of MDA were decreased significantly (P<0.05 or P<0.01). Conclusion: The classical prescription DSS could prevent and treat myocardial ischemia injure effectively

protect the myocardial cell.