Retrieved and inspected the 37 published articles of recent years on ‘Eighteen Incompatible Medicaments’ of Chinese herbs and CYP450 and analyzed the current situation on the impact of ‘Eighteen Incompatible Medicaments’ of Chinese herbs based on the CYP450. The methods in vivo and in vitro for studying the impact of ‘Eighteen Incompatible Medicaments’ on the CYP450 were put forward and the limitations of present research in methods. The in vitro study can not completely reflect in vivo and clinical research and concentration determination of mRNA of CYP450 can not overall reflect its activity were proposed. Some prospects in the study were given in order to discuss the scientific nature of ‘Eighteen Incompatible Medicaments’based on the CYP450. Both in vivo and in vitro experiments should be conducted and the effect of toxic ingredients metabolism on the production and enhancement of toxicity should be considered. These data may supply some references for research of the mechanisms of ‘Eighteen Incompatible Medicaments’.