Simultaneous Determination the Contents of Protodioscin, Methylprotodioscin, Pseudoprotodiosc in Rhizoma Dioscoreae Nipponicae with RP-HPLC

YU Shu-yi; LIU Ying; LIU Shu-min

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Simultaneous Determination the Contents of Protodioscin, Methylprotodioscin, Pseudoprotodiosc in Rhizoma Dioscoreae Nipponicae with RP-HPLC

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- Simultaneous Determination the Contents of Protodioscin, Methylprotodioscin, Pseudoprotodiosc in Rhizoma Dioscoreae Nipponicae with RP-HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 9, Pages: 96-98(2012)
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- Published：2012
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YU Shu-yi, LIU Ying, LIU Shu-min. Simultaneous Determination the Contents of Protodioscin, Methylprotodioscin, Pseudoprotodiosc in Rhizoma Dioscoreae Nipponicae with RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 96-98.
DOI：

YU Shu-yi, LIU Ying, LIU Shu-min. Simultaneous Determination the Contents of Protodioscin, Methylprotodioscin, Pseudoprotodiosc in Rhizoma Dioscoreae Nipponicae with RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 96-98. DOI：

摘要

目的: 建立测定产地黑龙江大兴安岭穿山龙供试品中水溶性皂苷含量的方法。方法: 采用RP-HPLC

Diamonsil (钻石) C18色谱柱(4.6 mm×250 mm

5 μm)

流动相乙腈-水梯度洗脱

检测波长203 nm

柱温30 ℃。结果: 原薯蓣皂苷浓度在50~500 mg·L-1、甲基原薯蓣皂苷在35~350 mg·L-1、伪原薯蓣皂苷质量浓度在15~150 mg·L-1与峰面积呈良好的线性关系

r=0.999 9(n=6)。方法学考察中各项实验结果的RSD均<3%。结论: 方法重复性好

具有良好的回收率

稳定性佳

为穿山龙药材的质量控制提供了参考。

Abstract

Objective :Establish a method for determining the content of water soluble saponins of Rhizoma Dioscoreae Nipponicae which grow in Daxinganling area of Heilongjiang Province. Method: RP HPLC was used on an Diamonsil C18 column(4.6 mm×250 mm

5 μm) with acetomitrile-water as mobile phase by gradient elution for the separation and determination of saponins of Rhizoma Dioscoreae Nipponicae

the detection wavelength was at 203 nm

column temperature 30 ℃. Result :The linear ranges were 50-500 mg·L-1 for protodioscin concentration

35-350 mg·L-1 for methyl protodioscin concentration

15-150 mg·L-1 for pseudoprotodioscin concentration

the calibration curve of each solvent had good linear relationship within the range with a correlation coefficient of 0.999 9(n=6). On the basis of methodology of the test results show RSD <3%. Conclusion :All the results show that the method has good reproducibility

recovery rate and stability. This has provided a basis for Rhizoma Dioscoreae Nipponicae quality control.

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Related Author

YU Shu-yi1

LIU Ying2

LIU Shu-min2

Shuai WANG

Lili WANG

Juanjuan FANG

Kunlin LIU

Yingzi WANG

Related Institution

First Affiliated Hospital Jiamusi University Jiamusi Municipality of Heilongjiang Province

Institute of Traditional Chinese Medicine Heilongjiang University of Chinese Medicine

School of Pharmacy，Shandong University of Traditional Chinese Medicine

Shandong Hongjitang Pharmaceutical Group Co. Ltd.

Shandong Center for Food and Drug Evaluation&Inspection

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