Objective: To establish the method for simultaneous determination of four active components in Baihu Decoction. Method: Simultaneous determination of neomangiferin

mangiferin

liquiritin and ammonium glycyrrhizinate in Baihu Decoction by HPLC. An Alltima C18 (4.6 mm×250 mm

5 μm) column was used

the mobile phase was acetonitrile and 25 mmol·L-1 of KH2PO4 solution in gradient elution

the detection wavelength was set at 256 nm

the column temperature was kept at 25 ℃ and the flow rate was 1 mL·min-1. Result: The linear range of neomangiferin

mangiferin

liquiritin and ammonium glycyrrhizinate was 0.15-3.00 μg (r=0.999 9)

0.10-2.00 μg (r=0.999 9)

0.04-0.80 μg (r=0.999 9)

0.04-0.80μg (r=0.999 9) separately. The average recovery was between 95.0%-104.5% for four ingredients. Conclusion: The method is convenient

stable

reliable and suitable for quality control of Baihu decoction.