Determination of the Content of Quercetin and Kaempferol from Different Medicinal Parts of  by HPLC

ZHU Xiao-qin; PENG Shui-mei; WU Jin-zhong

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Determination of the Content of Quercetin and Kaempferol from Different Medicinal Parts of by HPLC

更新时间：2024-01-04

- Determination of the Content of Quercetin and Kaempferol from Different Medicinal Parts of by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 10, Pages: 80-83(2012)
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- Published：2012
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ZHU Xiao-qin, PENG Shui-mei, WU Jin-zhong. Determination of the Content of Quercetin and Kaempferol from Different Medicinal Parts of by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(10): 80-83.
DOI：

ZHU Xiao-qin, PENG Shui-mei, WU Jin-zhong. Determination of the Content of Quercetin and Kaempferol from Different Medicinal Parts of by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(10): 80-83. DOI：

摘要

目的: 建立高效液相色谱法同时测定截叶铁扫帚不同药用部位中槲皮素、山奈酚含量的方法。方法: 采用Daisogel Sp-ODS-BP C18色谱柱(4.6 mm×250 mm

5 μm)

流动相甲醇-0.2%磷酸(63∶37)

流速1.0 mL·min-1

柱温25 ℃

检测波长360 nm。结果: 槲皮素在6.25~100 mg·L-1呈良好的线性关系(r=0.999 9)

加样回收率100.75%

RSD 1.92%

山奈酚在0.638~20.4 mg·L-1呈良好的线性关系(r=0.999 9)

加样回收率为99.76%

RSD 1.99%。根、枝、叶部位槲皮素的平均质量分数分别为9.00

41.79

221.86 μg·g-1

山奈酚的平均质量分数分别为3.09

7.52

40.72 μg·g-1。结论: 该方法简便快速

结果准确可靠

可作为截叶铁扫帚不同药用部位的含量测定方法

为合理开发利用截叶铁扫帚资源提供理论依据。

Abstract

Objective: To develop a HPLC method for the determination of the content of quercetin and Kaempferol from different medicinal parts of Lespedeza cuneata. Method: Samples were analyzed on Daisogel Sp-ODS-BP C18(4.6 mm×250 mm

5 μm)

with the mobile phase consisted of methanol-0.20% phosphoric acid solution (63∶37). The flow rate was 1.0 mL·min- 1

column tomparature was at 25 ℃. The UV wavelength was set at 360 nm. Result: The linear range of quercetin was 6.25-100 mg·L-1. The average recovery was 100.75% and RSD was 1.92%). The linear range of kaempferol was 0.638-20.4 mg·L-1.The average recovery was 99.76% and RSD was 1.99%. The concent of quercetin in root

branch and leaf was 9.00

41.79

221.86 μg·g-1. The concent of kaempferol was 3.09

7.52

40.72 μg·g-1. Conclusion: The method is simple

rapid

accurate and reliable. It can be used as a content determination method for the medicinal parts of L. cuneata. It can provide evidence for the rational development and utilization of L. cuneata.

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Related Author

ZHU Xiao-qin1

PENG Shui-mei2

WU Jin-zhong1

LIANG Chen-yan

ZHEN Han-shen

MA Wen-Fang

LIU Yuan-yuan

CHEN Mei-an

Related Institution

Academy of Integrative Medicine,Fujian University of Traditional Chinese Medicine

College of Pharmacy,Fujian University of Traditional Chinese Medicine

School of Life Sciences,Guizhou Normal University

Tianjin University of Chinese Medicine

Xiyuan Hospital， China Academy of Chinese Medical Sciences

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