Objective: To determine norswertianolin of Gentianella acuta by reverse-phase HPLC method. Method: The determination of norswertianolin was performed on a shim-pack VP-ODS-C18 column (4.6 mm ×150 mm

5 μm). The mobil phase was 0.1% phosphoric acid solution (A)-acetonitrile (B) (80: 20). The flow rate was 1.0 mL·min-1. Ultraviolet absorption detector was set at 254 nm and column temperature at 25 ℃. Norswertianolin of G. acuta was separated well. Result: The result indicated that the linear range of norswertianolin of G. acuta was between 9.6-192 mg·L-1(r=0.999 5); the relative standard deviations of instrument precision was 0.67%(n=6); the relative standard deviations of method reproducibility was 1.88% (n=6); the relative standard deviations for stability was 1.68%; the average recovery was 99.8% (n=6). Conclusion: The results indicated that this developed assay method was rapid

accurate

and reliable and could be readily used as a quality control method for the norswertianolin.