Objective: To establish a new RP-HPLC method for simultaneous determination of salvianolic acid A

salvianolic acid B

tanshinol

lithospermum acid

tanshinone ⅡA in crude extract of Radix Salvin Miltiorrhiza. Method: Analysis was carried out on a Kromasil 100-5 C18 (4.6 mm×250 mm

5 μm) column with acetonitrile-pure water(added 0.1% formic acid) as mobile phases in gradient elution. The flow rate was 1 mL·min-1

and the UV detector was monitored at 285 nm. Result: All calibration curve showed good linear regression in test ranges(r>0.999 5); and the overall recoveries were in the range of 97%-103%(n=3)with RSD less than 3%. The overall RSD of precision test were less than 3%. Conclusion: The method is rapid

accurate

credible and repeatable

and can provide bases for the material basis and compatibility for Radix Salvin Miltiorrhiza.