Separation and Purification Technology of Total Saponins fromby Macroporous Resin

HAN Jing; FENG Li-jun; YAN Lei; LIN Long-fei; NI Jian

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Separation and Purification Technology of Total Saponins fromby Macroporous Resin

更新时间：2024-01-04

- Separation and Purification Technology of Total Saponins fromby Macroporous Resin
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 10, Pages: 66-68(2012)
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- Published：2012
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HAN Jing, FENG Li-jun, YAN Lei, et al. Separation and Purification Technology of Total Saponins fromby Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(10): 66-68.
DOI：

HAN Jing, FENG Li-jun, YAN Lei, et al. Separation and Purification Technology of Total Saponins fromby Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(10): 66-68. DOI：

摘要

目的: 研究大孔吸附树脂法纯化西洋参总皂苷的工艺条件及参数。方法: 以西洋参总皂苷含量为指标

考察上样液质量浓度、洗脱溶媒及其用量、除杂溶媒及其用量等条件

优选大孔树脂纯化工艺条件。结果: 西洋参总皂苷最佳纯化工艺为0.8 BV上样液(0.5 g·mL-1)通过HPD-300大孔树脂

4 BV水洗

3 BV 10%乙醇洗脱

5 BV 70%乙醇洗脱

收集70%乙醇洗脱液

以人参皂苷Rg1、人参皂苷Rb1和人参皂苷Re计总皂苷纯度为50.83%。结论: 采用该优选方法可较好地分离、纯化西洋参总皂苷。

Abstract

Objective: To optimize purification technology parameters of total saponins from Panax quinquefolium by macroporous resin. Method: With the content of total saponins as index

purification technology was optimized by investigating the concentration of sample liquid

elution solvent and the amount of it

impurity solvent and the amount of it

et al. Result: Optimum purification technology was:0.8 BV sample liquid(0.5 g·mL-1) passed HPD-300 resin

eluted by 4 BV water and 3 BV 10% ethanol

eluted by 5 BV 70% ethanol

collected eluate

purity of total saponins was up to 50.83%(sum of ginseng saponin Rg1

ginseng saponin Rb1 and ginseng saponin Re). Conclusion: This method had a better separation and purification capacity for total saponins for Panax quinquefolium.

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Related Author

HAN Jing

FENG Li-jun

YAN Lei

LIN Long-fei

NI Jian

FENG Su-xiang

MIAO Ming-san

MIAO Jin-xin

Related Institution

College of pharmacy,Beijing University of Chinese Medicine

School of Chinese Materia Medica,Haerbin University of Commerce,Haerbin

College of Traditional Chinese Medicine,Hebei United University

School of Chinese Materia Medica,Beijing University of Chinese Medicine

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

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