Objective:To establish an HPLC method for content determination of loureirin A

B and 7

4'-dihydroxy flavone in extract of resina draconis. Method:Kromasil C18 column (4.6 mm ×250 mm

5 μm) was used with a step gradient of acetonitrile-1%glacial acetic acid at a flow rate of 1.0 mL ·min-1.The sample amount was 10 μL and the column temperature was maintained at 40 ℃. The detection wave length of loureirin A

B was 278 nm and 7

4'-dihydroxy flavone was 330 nm. Result:There was good linear relationship in the range of 10.05-60.36 μg for loureirin A(r=0.999 8)

10.08-60.48 μg for loureirin B(r=0.999 8) and 3.26-19.56 μg for 7

4'-dihydroxy flavone(r=0.999 8). The average recovery of loureirin A was 108.6% with relative standard deviation(RSD) of 0.99%

loureirin B was 109.6% with RSD of 1.03%

and 7

4'-dihydroxy flavone was 102.5% with RSD of 1.24%. Conclusion:This method is simple

accurate and can be used for quality control of extract of resina draconis.