Protection of Baicalein against Four Hepatotoxins-induced Cytotoxicity

JIANG Ping; YANG Yang; JI Li-li; WANG Zheng-tao

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Protection of Baicalein against Four Hepatotoxins-induced Cytotoxicity

更新时间：2024-01-04

- Protection of Baicalein against Four Hepatotoxins-induced Cytotoxicity
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 6, Pages: 145-148(2012)
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- Published：2012
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JIANG Ping, YANG Yang, JI Li-li, et al. Protection of Baicalein against Four Hepatotoxins-induced Cytotoxicity[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(6): 145-148.
DOI：

JIANG Ping, YANG Yang, JI Li-li, et al. Protection of Baicalein against Four Hepatotoxins-induced Cytotoxicity[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(6): 145-148. DOI：

摘要

目的: 观察黄芩素对4种肝毒性物质诱导的人正常肝细胞株L-02细胞损伤的拮抗作用。方法: 培养的L-02细胞分别给予10 mmol·L-1对乙酰氨基酚(AP)

50 μmol·L-1山岗橐吾碱(clivorine

CLI)

2 μmol·L-1川楝素(toosendanin

TSN)和100 mmol·L-1乙醇(EtOH)诱导肝细胞毒性。黄芩素1

100 μmol·L-1分别与细胞预孵15 min后

加入肝毒性物质

48 h后MTT法检测细胞存活率。结果 :与对照组相比

4种肝毒性物质均能显著降低肝细胞存活率(P<0.001)。经不同浓度黄芩素处理后

各浓度黄芩素均能显著提高AP

TSN

EtOH肝细胞损伤模型组的细胞存活率(P<0.01

P<0.001);10

100 μmol·L-1黄芩素能显著提高CLI肝细胞损伤模型组的细胞存活率(P<0.05

P<0.001)。结论 :黄芩素具有拮抗对乙酰氨基酚、山岗橐吾碱、川楝素和乙醇这些肝毒性物质诱导的肝细胞毒性的作用

且具有一定的浓度依赖性。

Abstract

Objective: The present study is designed to observe the protective effects of baicalein against hepatotoxins-induced cytotoxicity on the human normal liver L-02 cells. Method: L-02 cells were seeded and pretreated with or without baicalein (1

100 μmol·L-1) for 15 min

and then cells were further treated with or without various hepatotoxins

including acetaminophen (AP

10 mmol·L-1)

clivorine (CLI

50 μmol·L-1)

toosendanin (TSN

2 μmol·L-1) and ethanol (EtOH

100 μmol·L-1) for 48 h. The survival cells were determined by MTT assay. Result: Four hepatotoxins could significantly reduce the cell viability (P< 0.001) compared with the control group. After treatments

baicalein could increase the cell viability which was decreased by AP

TSN and EtOH (P<0.01

P<0.001). Moreover

baicalein could significantly increase the cell viability which was decreased by CLI (P<0.05

P<0.001). Conclusion: Baicalein can prevent the cytotoxicity induced by those hepatotoxins in human normal liver L-02 cells.

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