Objective: To evaluate the preventive and therapeutic effects of Shufeng Jiedu granules on hand-foot-mouth disease by observing the death protective effect of the granules on suckling BALB/c mice infected by enterovirus 71 in vivo and the inhibition effect on enteric viruses in vitro. Method: Prophylactic and therapeutic administration of Shufeng Jiedu granules were applied to EV71 H strain infection suckling BALB/c mice at 2

1 and 1/2 clinical equivalent dose of 3-year-old children respectively. We observed the degree of infection

death number and calculated the death rates and life extension rates. The in vitro inhibition of Shufeng Jiedu granules at five non-toxic dosages anti EV71 H strain

CoxB3 strain

CoxB4 strain and CoxB5 strain were determined by CPE method on Vero cells. Result: Shufeng Jiedu granules could decrease the degree of infection and death rates

simultaneously increase life extension rates significantly. Shufeng Jiedu granules showed significant preventive and therapeutic effects on suckling BALB/c mice infected by enterovirus 71.At no-toxic dosages

Shufeng Jiedu granules inhibited CoxB3 strain

CoxB4 strain and CoxB5 strain visibly. Conclusion: Shufeng Jiedu granules show notable preventive and therapeutic effects on hand-foot-mouth disease in vivo and in vitro.