Determination for Content of Gentiopicroside, Naringin and Protosappanin B from Xuanbi Gel Plaster

LIU Yue-huan; DU Shou-ying; LU Yang; BAI Jie; WANG Yue; LI Hui-yun

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Determination for Content of Gentiopicroside, Naringin and Protosappanin B from Xuanbi Gel Plaster

更新时间：2024-09-23

- Determination for Content of Gentiopicroside, Naringin and Protosappanin B from Xuanbi Gel Plaster
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 9, Pages: 72-74(2012)
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- Published：2012
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LIU Yue-huan, DU Shou-ying, LU Yang, et al. Determination for Content of Gentiopicroside, Naringin and Protosappanin B from Xuanbi Gel Plaster[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 72-74.
DOI：

LIU Yue-huan, DU Shou-ying, LU Yang, et al. Determination for Content of Gentiopicroside, Naringin and Protosappanin B from Xuanbi Gel Plaster[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 72-74. DOI：

摘要

目的: 建立宣痹凝胶膏剂中龙胆苦苷、柚皮苷、原苏木素B含量测定方法。方法: 采用高效液相色谱法

Merck RP-C18色谱柱(4.6 mm× 250 mm

5 μm)

流动相乙腈-0.1%磷酸梯度洗脱(0~33 min

11%乙腈;33~36 min

11%乙腈~20%乙腈;36~67 min

20%乙腈)

柱温20 ℃

流速0.6 mL·min-1

检测波长283 nm

进样量10 μL。结果: 龙胆苦苷、柚皮苷、原苏木素B分别在0.014 9~0.745 0 μg

0.007 1~0.355 0 μg

0.002 2~0.107 5 μg呈良好的线性关系

平均回收率分别为98.87%(RSD 2.19%)

103.51%(RSD 0.94%)

99.58%(RSD 2.49%)。结论: 方法操作简便

准确

重复性好

可作为宣痹凝胶膏剂的质量控制。

Abstract

Objective: To establish content determination method for gentiopicroside

naringin and protosappanin B from Xuanbi gel plaster. Method: HPLC was used with Merck RP-C18 column (4.6 mm×250 mm

5 μm)

gradient eluted with mobile phase of 0.1% phosphoric acid-acetonitrile(0-33 min

11% acetonitrile; 33-36 min

11% acetonitrile-20% acetonitrile; 36-67 min

20% acetonitrile)

column temperature was 20 ℃

flow rate was 0.6 mL·min-1

detection wavelength 283 nm

injection volume was 10 μL. Result: The linear range of gentiopicroside

naringin and protosappanin B were 0.014 9-0.745 μg

0.007 1-0.355 μg

0.002 2-0.107 5 μg

respectively.Average recovery of these ingredients were 98.87% (RSD 2.19%)

103.51% (RSD 0.94%)

99.58% (RSD 2.49%)

respectively. Conclusion: This established method was simple

accurate and repeatable

it could be used as quality control method for Xuanbi gel plaster.

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Related Author

LIU Yue-huan

DU Shou-ying

LU Yang

BAI Jie

WANG Yue

LI Hui-yun

LOU Min1

ZHENG Peng2

Related Institution

School of Chinese Pharmacy,Beijing University of Chinese Medicine

Natural Pharmaceutical R&D

修正药业长春高新制药有限公司

吉林省中医药科学院

吉林天药本草堂制药有限公司

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