Protection of Puerarin on Oxidative Stress Induced by Acute Myocardial Ischemia-Reperfusion Injury in Rats in Cardiopulmonary Bypass
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Protection of Puerarin on Oxidative Stress Induced by Acute Myocardial Ischemia-Reperfusion Injury in Rats in Cardiopulmonary Bypass
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 1, Pages: 165-168(2012)
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Published:2012
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GONG Hong-yan, QIN Yuan-xu, WANG Geng-fu, et al. Protection of Puerarin on Oxidative Stress Induced by Acute Myocardial Ischemia-Reperfusion Injury in Rats in Cardiopulmonary Bypass[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(1): 165-168.
DOI:
GONG Hong-yan, QIN Yuan-xu, WANG Geng-fu, et al. Protection of Puerarin on Oxidative Stress Induced by Acute Myocardial Ischemia-Reperfusion Injury in Rats in Cardiopulmonary Bypass[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(1): 165-168.DOI:
Protection of Puerarin on Oxidative Stress Induced by Acute Myocardial Ischemia-Reperfusion Injury in Rats in Cardiopulmonary Bypass
随即阻断大鼠升主动脉造成心肌缺血30 min然后开放升主动脉后再灌注180 min造成大鼠心肌缺血再灌注损伤模型(灌注24 h
用于测定心肌梗死面积)。实验组和对照组分别给予葛根素和生理盐水。实验完成后留取大鼠心脏标本
观察大鼠心肌缺血区的心肌细胞凋亡情况;收集血清测定其抗氧化应激的指标:超氧化物歧化酶(SOD)
丙二醛(MDA)
谷胱甘肽(GSH)
谷胱甘肽过氧化物酶(GSH-Px)。 结果: 与模型组相比
葛根素的应用减少了MIRI大鼠的心肌细胞凋亡、心梗面积和血清中丙二醛的含量
增加了血清中超氧化物歧化酶、谷胱甘肽过氧化物酶的活性和谷胱甘肽的含量
并且随着剂量的增加保护效果尤为明显。 结论: 葛根素对MIRI大鼠具有抗氧化应激的作用
它能够剂量依赖性的减少心肌细胞凋亡
最终减少心肌梗死面积。
Abstract
Objective: To investigate the protective mechanism of puerarin on acute myocardial ischemia-reperfusion injury(MIRI)in rats
and to explain the antioxidative mechanism involved. Method: Rat MIRI model was induced by ischemia for 30 min and reperfusion for 180 min. At the end of the 3 h reperfusion period(or 24 h for infarct size)
myocardial infarct size
myocardial apoptosis and the activity of antioxidative enzymes were measured. Result: Puerarin reduced infarct size
myocardial apoptosisand the serum level of malondialdehyde
increased the activity of superoxide dismutase and glutathione-peroxidase activities and serum level of glutathione. Conclusion: Puerarin exerts significant cardioprotective effects against MIRI in rats
likely through its antioxidation and antilipid peroxidative properties.
Establishment and Evaluation of Rat Model of Myocardial Ischemia-reperfusion Injury with Phlegm and Blood Stasis Blocking Collaterals Syndrome Based on Metabolomics
Mechanism of Danshenol A in Alleviating Myocardial Ischemia-reperfusion Injury-induced Ferroptosis of Cardiomyocytes
Mechanism of Chinese Medicine Polysaccharide in Improving Myocardial Ischemia-reperfusion Injury
Mechanism of Prevention and Treatment of Myocardial Ischemia-reperfusion Injury by Qi-replenishing and Blood-activating Chinese Medicines Based on Theory of Qi and Blood Interacting in Vessels
Effect of Modified Shenhong Tongluo Prescription on Cell Apoptosis in Rats with Myocardial Ischemia-reperfusion Injury by Bcl-2/Bax/Caspase-3 Signaling Pathway
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