Objective: To establish a high performance liquid chromatographic method (HPLC) for sim ultaneous determination of piperine and piperlongumine in Piper longum. Method :Agilent Eclipse XDB-C18 column (4.6 mm×250 mm

5 μm) was used to separate the analytes using a mode with a mobile phase consisting of water with 0.25% formic acid (35) and methyl alcohol (65) at a flow rate of 1 mL ·min-1 and an operating column temperature of 40 ℃. The detection wave length was set at 343 nm. Result: All the constituents showed good linearity (r=0.999 9) in the range of the tested concentration

the average recoveries of the method were 100.4% and 102.2%

RSD were 2.6% and 2.5%.This method successfully determined 2 compounds in P. longum (from 8 different origins) simultaneously and quantitively. Conclusion: This is a more reasonable and credible quality control method for the Chinese traditional medicine of P. longum and it is simple

well reproducible.