Determination of Ursolic Acid and Oleanolic Acid in Zuogui Wan by HPLC

ZHANG Wen-jie; GUAN Da-ping; QIAN Shi-bing

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Determination of Ursolic Acid and Oleanolic Acid in Zuogui Wan by HPLC

更新时间：2024-01-04

- Determination of Ursolic Acid and Oleanolic Acid in Zuogui Wan by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 5, Pages: 92-94(2012)
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- Published：2012
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ZHANG Wen-jie, GUAN Da-ping, QIAN Shi-bing. Determination of Ursolic Acid and Oleanolic Acid in Zuogui Wan by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(5): 92-94.
DOI：

ZHANG Wen-jie, GUAN Da-ping, QIAN Shi-bing. Determination of Ursolic Acid and Oleanolic Acid in Zuogui Wan by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(5): 92-94. DOI：

摘要

目的: 建立HPLC测定左归丸中齐墩果酸、熊果酸含量的方法。方法: 采用反相高效液相色潽法

Sunfire C18柱

甲醇-水(85∶15)(磷酸二氢钠调pH 6.5)

流速1.0 mL·min-1

检测波长215 nm

柱温25 ℃。结果: 齐墩果酸在0.05~1.0 μg线性关系良好(r=0.999 9)

回收率97.7%(RSD 1.39%

n=6);熊果酸在0.1~2.0 μg线性关系良好(r=0.999 9)

回收率98.3%(RSD 0.82

n=6)。结论: 方法可用于左归丸中熊果酸、齐墩果酸的含量测定。

Abstract

Objective: To establish a HPLC method for the determination of ursolic acid and oleanolic acid in Zuogui Wan. Method: The method was applied with Sunfire C18 column by methanol-water (25∶75

adjust pH to 6.5 with sodium dihydrogen phosphate) as the mobile phase

with the flow rate 1.0 mL·min-1. The column temperature was maintained at 25 ℃ and the UV detection wavelength was set at 215 nm. Result: Oleanolic acid was linear in the range of 0.05-1.0 μg (r=0.999 9)

the average recovery was 97.7% (RSD 1.39

n=6). Ursolic acid was linear in the range of 0.1-2.0 μg (r=0.999 9)

the average recovery was 98.3% (RSD 0.82

n=6). Conclusion: The method can be used for determination of oleanolic acid and ursolic acid in Zuogui Wan.

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