Content Determination and Entrapment Efficiency Investigation of Gentiopicroside Lipsome

BAI Lan; ZHAO Ming-qin; YIN Rong-li; LI Dong-fen

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Content Determination and Entrapment Efficiency Investigation of Gentiopicroside Lipsome

更新时间：2024-01-04

- Content Determination and Entrapment Efficiency Investigation of Gentiopicroside Lipsome
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 7, Pages: 48-50(2012)
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- Published：2012
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BAI Lan, ZHAO Ming-qin, YIN Rong-li, et al. Content Determination and Entrapment Efficiency Investigation of Gentiopicroside Lipsome[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(7): 48-50.
DOI：

BAI Lan, ZHAO Ming-qin, YIN Rong-li, et al. Content Determination and Entrapment Efficiency Investigation of Gentiopicroside Lipsome[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(7): 48-50. DOI：

摘要

目的: 建立龙胆苦苷脂质体含量及包封率测定的方法。方法: 通过方法学考察

建立HPLC测定龙胆苦苷含量的方法;分别采用离心法和透析法从龙胆苦苷脂质体中分离龙胆苦苷

分别测定

计算包封率。结果: 确定龙胆苦苷脂质体含量测定条件为流动相甲醇-水(30:70)

流速1.0 mL·min-1

检测波长270 nm;离心法和透析法测得平均包封率分别为69.67%

76.12%。结论: 建立的HPLC条件可用于龙胆苦苷脂质体的含量测定

离心法测得的包封率较透析法小。

Abstract

Objective: To establish conditions for content determination and entrapment efficiency determination of gentiopicroside lipsome. Method: Establish a HPLC method for determination of the content of gentiopicroside by methodology investigation;Separated gentiopicroside from gentiopicroside lipsome by centrifugation and equilibrium dialysis method

and determined respectively

calculated entrapment efficiency. Result: HPLC conditions for determination of gentiopicroside lipsome were:mobile phase of methanol-water(30: 70)

flow rate 1.0 mL·min-1

detection wavelength 270 nm;The average entrapment efficiency with centrifugation was 69.67%

and it was 76.12% with equilibrium dialysis method. Conclusion: This established HPLC conditions could used for determination of the content of gentiopicroside lipsome

determined entrapment efficiency with centrifugation was less than equilibrium dialysis.

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