Objective: To establish the content determination of diosmin by UV-Vis spectrophotometry

and investigate solubilization of methyl-β-cyclodextrin (RM-β-CD) on diosmin. Method: Standard curve of content determination for diosmin by UV-Vis spectrophotometry was drew

and methodology was studied

Phase solubility method was adopted to investigate solubilization of RM-β-CD on diosmin

apparent stability constant (Kc) was calculated. Result: UV detection wavelength of diosmin was 372 nm

and there was a good linear relationship among 8-32 mg·L-1

solubility of diosmin increased linearly with improving of RM-β-CD concentration

phase solubility curve was classified as AL type

Kc was 111.09 mol·L-1. Conclusion: RM-β-CD could obviously increase solubility of diosmin.