Objective: To study the effect of Hepatitis B immunityglobulin(HBIG) with different injection methods to interrupt inner-uterus transmission from mothers with positive epatitis B surface antigen(HBsAg) and Hepatitis B Virus-DNA(HBV-DNA) serum to infants for the selection of the most effective and cheapest project. Method: One hundred and thirty-two pregnant women with positive HBsAg and HBV-DNA serum were randomly divided into four groups. 200 U HBIG was intramuscularly injected to 27 women of group A at 20

24

28

32

36

38 gestational weeks. 400 U HBIG was intramuscularly injected to 38 women of group B at 28

32

36 gestational weeks. 200 U HBIG was intramusculaly rinjected to 45 women of group C at 28

32

36 gestational weeks. There was no intervention in 22 women of control group. HBIG and HBVac were used to all the 132 newborns. Serum HBV-M and HBV-DNA of those infants were tested at 7 month of birth. Result: Intrauterine transmission rate of group A

B

C and control group was 7.4%

7.9%

11.1%

36.4%

separately. A significant difference was found between group A and control group

group B and control group

group C and control group(P<0.05). No significant difference was found among the interventional groups. Conclusion: It is effective and safe to use HBIG during pregnant. We can concluded that the method with 200 U injection 3 times can achieve the same effect with the other two methods (400 U 3 times

200 U 6 times). Obviously

it can be the cheapest project.