Objective: To explore the molecular mechanism of inhibiting colon cancer cell strein LoVo proliferation and inducing apoptosis by oxymatrine (OM) Method: Flow cytometry was used to detect the LoVo cells apoptosis and cell cycle distribution. Fluorescence quantitative PCR was used to detect cell proliferation-related genes like the c-myc

proteasome modulator 9(PSMD9)

CDK4 gene expression when LoVo was treated with 0.25

0.5 g·L-1OM. Result: OM had no significant effect on apoptosis in colon cancer LoVo cells when the treatment of OM lasted 48 h and the concentration was lower than 0.5

0.25 g·L-1 OM can inhibit c-myc gene expression in LoVo when duration of action last 24 h (P<0.05). When the dose increated to 0.5 g·L-1 and duration of action was 48 h

OM could inhibit c-myc

CDK4 gene expression in LoVo cells (P<0.01

P<0.01). When duration of action was extended to 96 h

0.5 g·L-1 OM could inhibit the c-myc

PSMD9

CDK4 gene expression in LoVo cells(P<0.05

P<0.01

P<0.01). Conclusion: OM at Lower dose could significantly inhibit the proliferation of human colon cancer LoVo cells

the mechanism may be related to reducing c-myc

PSMD9

CDK4 expression in LoVo cells.