Optimization of Flash-type Extraction Technology of Baicalin from by Response Surface Methodology
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Optimization of Flash-type Extraction Technology of Baicalin from by Response Surface Methodology
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 6, Pages: 48-51(2012)
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Published:2012
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FAN Cheng-jie, JIANG Dao-feng, LING Zong-shi. Optimization of Flash-type Extraction Technology of Baicalin from by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(6): 48-51.
DOI:
FAN Cheng-jie, JIANG Dao-feng, LING Zong-shi. Optimization of Flash-type Extraction Technology of Baicalin from by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(6): 48-51.DOI:
Optimization of Flash-type Extraction Technology of Baicalin from by Response Surface Methodology
单因素试验考察乙醇体积分数、提取时间及料液比;采用Box-Behnken设计、响应面分析统计学方法对工艺参数进行优化。 结果: 最佳工艺条件为14倍量70%乙醇提取102 s
在此条件下得到黄芩苷的实测值达13.588%
理论值可达13.592%
两者较吻合。 结论: 闪式提取法是一种高效、快速提取黄芩中黄芩苷的方法。
Abstract
Objective: To optimize flash-type extraction technology of baicalin from Scutellaria baicalensis by response surface methodology (RSM). Method: With yield of baicalin as index
the content of baicalin was determined by HPLC. Concentration of ethanol
extracting time and liquid-material ratio were selected by single-factor test;Technology parameters were optimized by Box-Benhnken design and response surface analysis statistical method. Result: Optimal extraction technology conditions were as follows:concentration of ethanol 73%
extraction time 102 s
liquid-solid ratio 14: 1 (mL: g)
under these optimum conditions
measured value of baicalin was 13.588%
it was in line with theoretical value of 13.592%. Conclusion: Flash-type extraction was proved to be a kind of efficient and rapid method for extraction of baicalin from S. baicalensis.
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