Optimization of Precipitation Desalination Technology for Regenenrated Silk Fibrion (RSF) by Orthogonal Test
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Optimization of Precipitation Desalination Technology for Regenenrated Silk Fibrion (RSF) by Orthogonal Test
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 6, Pages: 45-47(2012)
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Published:2012
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YU Li, WANG Hou-wei, XU Ling-chuan. Optimization of Precipitation Desalination Technology for Regenenrated Silk Fibrion (RSF) by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(6): 45-47.
DOI:
YU Li, WANG Hou-wei, XU Ling-chuan. Optimization of Precipitation Desalination Technology for Regenenrated Silk Fibrion (RSF) by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(6): 45-47.DOI:
Optimization of Precipitation Desalination Technology for Regenenrated Silk Fibrion (RSF) by Orthogonal Test
Objective: To optimize precipitation desalination technology for regeneration silk fibroin by orthogonal test. Method: Application of neutral calcium chloride prepared regenerated silk fibroin solution
desalination was applied by ethanol precipitation
desalination technology was optimized by orthogonal test with investigating factors(the concentration of ethanol
the amount of ethanol
precipitation time) of affecting precipitation process parameters for fibroin protein protein. Result: Optimum precipitation desalination technology for regeneration silk fibroin was:precipitated 4 h with 6 times the amount of 100%ethanol
at this point the concentration of silk fibroin solution was 0.08 g·mL-1. Precipitation desalination efficiency could reach 97.34%
yield of silk fibroin protein was 77.28%. Conclusion: This methord was stable and feasible
it had high desalination efficiency and could be used in desalination technology of protein.
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