HPLC Determination of the Content of Oleanolic Acid 3-phthalic Monoester Disodium Salt and its Related Substances

MA Xiu-ping; JIANG Zhao-hui; JIA Xian-sheng; CHEN Cong; DEN Hua-xiang

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HPLC Determination of the Content of Oleanolic Acid 3-phthalic Monoester Disodium Salt and its Related Substances

更新时间：2024-01-04

- HPLC Determination of the Content of Oleanolic Acid 3-phthalic Monoester Disodium Salt and its Related Substances
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 2, Pages: 89-91(2012)
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- Published：2012
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MA Xiu-ping, JIANG Zhao-hui, JIA Xian-sheng, et al. HPLC Determination of the Content of Oleanolic Acid 3-phthalic Monoester Disodium Salt and its Related Substances[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(2): 89-91.
DOI：

MA Xiu-ping, JIANG Zhao-hui, JIA Xian-sheng, et al. HPLC Determination of the Content of Oleanolic Acid 3-phthalic Monoester Disodium Salt and its Related Substances[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(2): 89-91. DOI：

摘要

目的: 用高效液相色谱法测定齐酞酸钠的含量及其有关物质。方法: 采用 Eclipse XDB-C18色谱柱(4.6 mm×150 mm

5 μm)

以甲醇-乙腈-0.1%磷酸水溶液(80∶10∶10)为流动相

流速1.0 mL·min-1

柱温35 ℃

检测波长210 nm。结果: 齐酞酸钠与其相邻杂质峰能完全分离

齐酞酸钠在0.052 5~0.997 5 μg进样量与峰面积呈良好线性关系(r=0.999 9)

最低检出限为3 ng;精密度良好(RSD 0.38%)

平均回收率为99.53%(n=9)。结论: 该方法简便、准确、专属性好、灵敏度高

可用于齐酞酸钠的含量及有关物质测定。

Abstract

Objective: To determine the content and the related substances of oleanolic acid 3β-phthalic monoester disodium salt (OAPES) by HPLC. Method: The separation was performed on Eclipse XDB-C18 column(4.6 mm×150 mm

5 μm)

column temperature at 35 ℃ with mobile phase of methanol-acetonitrile-0.1% phosphoric acid solution(80∶10∶10). The flow rate was set at 1.0 mL·min-1 with detection at 210 nm. Result: There was a good linear relationship within the range of 0.052 5-0.997 5 μg(r=0.999 9)

the least detective range is 3 ng. The precision in a day is good (RSD 0.38%). The average recovery was 99.53%(n=9). Conclusion: The method is simple

accurate

specific and sensitiveness for the determination OAPES content and its related substances.

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